rsiX [2019-06-06 12:19:56]

anti-SigX

Locus: BSU23090

Isoelectric point: 5.45

Molecular weight: 41.15 kDa

Protein length: 368 aa

Gene length: 1107 bp

Function: control of SigX activity

Product: anti-SigX

Essential: no

Synonyms: ypuN

Genomic Context

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Categories containing this gene/protein

• Information processing → Regulation of gene expression → Sigma factors and their control → Control of sigma factors
• Lifestyles → Coping with stress → Cell envelope stress proteins (controlled by SigM, V, W, X, Y)
• Groups of genes → Membrane proteins

Gene

Coordinates: 2,413,585 2,414,691

Phenotypes of a mutant

• inactivation of rsiX strongly restores beta-lactam resistance in a sigM mutant PubMed

The protein

Localization

• cell membrane

Expression and Regulation

Operons

• Genes: sigX-rsiX
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigX: sigma factor, PubMed, in SigX regulon

  Regulatory mechanism

  • YvrHb: activation, PubMed, in YvrHb regulon

  Regulation

  • induced by glucose PubMed, this depends on YlxR PubMed

Biological materials

Mutant

• MGNA-A180 (ypuN::erm), available at the NBRP B. subtilis, Japan
• BKE23090 (rsiX::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AATATCCTGAGGCGAACGAT, downstream forward: _UP4_TAAAAAGAGCCCTTTAAGGC
• BKK23090 (rsiX::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AATATCCTGAGGCGAACGAT, downstream forward: _UP4_TAAAAAGAGCCCTTTAAGGC

References

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