rsiX [2019-06-06 12:19:56]

anti-SigX

locus: BSU23090

pI: 5.45

mw: 41.15 kDa

protein length: 368 aa

gene length: 1107 bp

function: control of SigX activity

product: anti-SigX

essential: no

synonyms: ypuN

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.2|Control of sigma factors]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 2,413,585 2,414,691

Phenotypes of a mutant

• inactivation of ''[gene|21D260E900776EAAF4B6000A3365DCA9241EACA6|rsiX]'' strongly restores beta-lactam resistance in a ''[gene|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]'' mutant [Pubmed|22211522]

The protein

[SW|Localization]

• cell membrane

Expression and Regulation

Operons

• genes: [gene|E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX]-[gene|21D260E900776EAAF4B6000A3365DCA9241EACA6|rsiX]
  description: [Pubmed|7934830]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9636707], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX]: sigma factor, [Pubmed|9636707], in [regulon|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX regulon]

  regulatory mechanism

  • [protein|7098319D8E88FDC2A629A3F4A21507FD7A649385|YvrHb]: activation, [Pubmed|16306698], in [regulon|7098319D8E88FDC2A629A3F4A21507FD7A649385|YvrHb regulon]

  regulation

  • induced by glucose [Pubmed|27965645], this depends on [protein|F4097349A563503468A2A14F062AEAC532C7917A|YlxR] [pubmed|30355672]

Biological materials

Mutant

• MGNA-A180 (ypuN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/180 NBRP B. subtilis, Japan]
• BKE23090 ([gene|21D260E900776EAAF4B6000A3365DCA9241EACA6|rsiX]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATATCCTGAGGCGAACGAT, downstream forward: _UP4_TAAAAAGAGCCCTTTAAGGC
• BKK23090 ([gene|21D260E900776EAAF4B6000A3365DCA9241EACA6|rsiX]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATATCCTGAGGCGAACGAT, downstream forward: _UP4_TAAAAAGAGCCCTTTAAGGC

References

• 14993308,7934830,16306698,9636707,9393439,22211522